Description
In enzymology, a NADPH dehydrogenase (EC 1.6.99.1) is an enzyme that catalyzes the chemical reaction: NADPH + H+ + acceptor ↔ NADP+ + reduced acceptor. The 3 substrates of this enzyme are NADPH, H+, and acceptor, whereas its two products are NADP+ and reduced acceptor. This enzyme belongs to the family of oxidoreductases, specifically those acting on NADH or NADPH with other acceptors.
Abbr
Diaphorase (NADPH), Native (Bacillus megaterium)
Source
Bacillus megaterium
Applications
Useful for enzymatic determination of reduced NADP.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.6.99.1
Activity
More than 5 U/mg solid
Contaminants
Myokinase < 0.50%
Molecular Weight
48 kDa (gel filtration)
Michaelis Constant
NADPH 2.9 × 10-4M
Unit Definition
One unit is defined as the amount of enzyme which oxidizes 1 μmole of NADPH to NADP+ per minute at 30°C under the conditions specified in the assay procedure.
Thermal stability
Stable at 60°C and below
Storage
At least one year at -20°C
Synonyms
NADPH:acceptor oxidoreductase; NADPH2 diaphorase; NADPH diaphorase; OYE; diaphorase; dihydronicotinamide adenine dinucleotide phosphate dehydrogenase; NADPH-dehydrogenase; NADPH-diaphorase; NADPH2-dehydrogenase; old yellow enzyme; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; TPNH dehydrogenase; TPNH-diaphorase; triphosphopyridine diaphorase; triphosphopyridine nucleotide diaphorase; NADPH2 dehydrogenase; NADPH: (acceptor) oxidoreductase; NADPH dehydrogenase; EC 1.6.99.1
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