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Diaphorase 22 from Recombinant E.coli
Cat No.
NATE-1938
Description
Recombinant Diaphorase 2.2 from E. coli for advanced research on redox reactions and enzymatic activity. Ideal for biochemistry and molecular biology studies. Creative Enzymes ensures high-purity, reliable solutions.
Source
E. coli
Form
Lyophilized
Enzyme Commission Number
EC 1.8.1.4
Activity
>150 U/mg protein
CAS No.
9001-18-7
Contaminants
(as Diaphorase activity = 100 %)
Adenylate kinase < 0.01 %
NADH oxidase < 0.20 %
Adenylate kinase < 0.01 %
NADH oxidase < 0.20 %
Molecular Weight
ca. 110,000
pH Stability
6.0 – 9.0
Michaelis Constant
(50 mM HEPES buffer, pH 7.0, at 30 °C)
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) 0.345 mM
NADH 0.033 mM
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) 0.345 mM
NADH 0.033 mM
Unit Definition
One unit of activity is defined as the amount of Diaphorase that forms 1 μmol of NAD+ per minute at 30 °C.
Optimum pH
8
Thermal stability
No detectable decrease in activity up to 70 °C.
Storage
Store at -20°C
Synonyms
LDP-Glc; LDP-Val; dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; protein-6-N-(dihydrolipoyl)lysine:NAD+ oxidoreductase
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Download Datasheet"Diaphorase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1927 | Diaphorase-3, recombinant | Inquiry | |||
| NATE-1901 | Native Bacillus stearothermophilus Diaphorase 1 | EC 1.6.99.- | Bacillus stearothermophilus | Inquiry | |
| DIA-320 | Diaphorase from E. coli, Recombinant | EC 1.8.1.4 | 9001-18-7 | E. coli | Inquiry |
| NATE-1154 | Native Diaphorase (NADPH) from Bacillus megaterium | EC 1.6.99.1 | 9001-68-7 | Bacillus megaterium | Inquiry |