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Native Clostridium sp. Diaphorase

Cat No.
DIA-187
Abbr
Diaphorase (Clostridium sp.)
Alias
Diaphorase
Source
Clostridium sp.
Applications
This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
Appearance
Yellowish amorphous powder, lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 1.6.99.-
Activity
GradeⅢ 30U/mg-solid or more (containing approx. 15% of stabilizers)
Contaminants
Myokinase < 5.0×10⁻¹% NAD(P)H oxidase < 5.0×10⁻¹%
Molecular Weight
24 kDa
pH Stability
pH 7.5 (30°C, 3hr)
Michaelis Constant
2.0×10⁻⁵M (NADH), 6.0×10⁻⁶M (NADPH)
Structure
One mol of FMN per mol of enzyme
Specificity
Either NADH or NADPH can be used as a reductant. The catalytic ratio (NADPH/NADH) is 0.6 in the assay method. Neither oxygen nor cytochrome C can be utilized as a hydrogen acceptor.
Optimum pH
8.5
Optimum temperature
50°C
Thermal stability
below 30°C (pH 7.5, 30min)
Stability
Stable at-20°C for at least one year
Stabilizers
FMN, NAD(P)H
Inhibitors
N-Ethylmaleimide
Synonyms
Diaphorase; EC 1.6.99.-

"Diaphorase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1927 Diaphorase-3, recombinant Inquiry
NATE-1901 Native Bacillus stearothermophilus Diaphorase 1 EC 1.6.99.- Bacillus stearothermophilus Inquiry
DIA-320 Diaphorase from E. coli, Recombinant EC 1.8.1.4 9001-18-7 E. coli Inquiry
NATE-1938 Diaphorase 22 from Recombinant E.coli EC 1.8.1.4 9001-18-7 E. coli Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

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