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Native Bovine Deoxyribonuclease I

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Cat No.

PHAM-266

Description

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.2 The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.3 A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with molar ratios being 4:1:1 respectively. Only minor amounts of D are found.4 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)5 and actin6 are known to inhibit the enzyme activity.

Abbr

DNase I, Native (Bovine)

Alias

DNase I

Source

Bovine pancreas

Species

Bovine

Applications

Used for the removal of DNA from protein samples.

Package

vial of >0.5 mg total protein

Enzyme Commission Number

EC 3.1.21.1

CAS No.

9003-98-9

Molecular Weight

mol wt ~31 kDa

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM

Storage

−20°C

Synonyms

EC 3.1.21.1; DNase I; Deoxyribonuclease I; Deoxyribonucleate 5'-oligonucleotido-hydrolase

"DNASE1" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1875	Native Bovine Deoxyribonuclease I	EC 3.1.21.1	9003-98-9	Bovine Spleen	Inquiry
NATE-0198	Native Bovine Deoxyribonuclease I	EC 3.1.21.1	9003-98-9	Bovine pancreas	Inquiry
NATE-0199	Native Bovine Deoxyribonuclease I RNase-free solution	EC 3.1.21.1	9003-98-9	Bovine pancreas	Inquiry
NATE-0200	Deoxyribonuclease I from Bovine, Recombinant	EC 3.1.21.1	9003-98-9	Pichia pastoris	Inquiry

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