Description
In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + diphosphate + NAD+. The 3 substrates of this enzyme are ATP, deamido-NAD+, and NH3, whereas its 3 products are AMP, diphosphate, and NAD+. This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). This enzyme participates in nicotinate and nicotinamide metabolism and nitrogen metabolism.
Abbr
NAD Synthetase, Native (Bacillus stearothermophilius)
Source
Bacillus stearothermophilius
Applications
Useful for enzymatic determination of ATP, ammonia, urea or creatinine. It is also suitable for enzymatic cycling method
Product Overview
Is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Enzyme Commission Number
EC 6.3.1.5
Molecular Weight
50 kDa (gel filtration); 25 kDa (SDS‒PAGE)
Isoelectric point
pH 5.2 ± 0.2
pH Stability
6.0-9.0 (37°C, 15 mins)
Michaelis Constant
Deamido‒NAD 2.4 × 10-5M; ATP 4.3 × 10-5M; NH3 2.16 × 10-3M
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of deamido‒NAD to NAD+ per minute at 37°C under the conditions specified in the assay procedure.
Optimum temperature
70°C (Tris‒HCl buffer)
Thermal stability
Stable at 60°C and below (pH 7.5, 10 mins)
Storage
Storage at-20°C in the presence of a desiccant is recommended.
Synonyms
EC 6.3.1.5; 9032-69-3; NAD+ synthetase; NAD+ synthase; nicotinamide adenine dinucleotide synthetase; diphosphopyridine nucleotide synthetase
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