Description
In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + diphosphate + NAD+. The 3 substrates of this enzyme are ATP, deamido-NAD+, and NH3, whereas its 3 products are AMP, diphosphate, and NAD+. This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). This enzyme participates in nicotinate and nicotinamide metabolism and nitrogen metabolism.
Abbr
NAD Synthetase, Recombinant (B. subtilis)
Form
Liquid. In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT.
Activity
~0.3 U/mg protein
Molecular Weight
~30.4kDa
Concentration
0.5mg/ml (Lot specific)
Unit Definition
One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.
Stability
Stable for at least 6 months after receipt when stored at -80°C.
Storage
Store at -20°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Synonyms
EC 6.3.1.5; 9032-69-3; NAD+ synthetase; NAD+ synthase; nicotinamide adenine dinucleotide synthetase; diphosphopyridine nucleotide synthetase
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