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Native Bacillus sp. Leucine dehydrogenase

Cat No.
DIA-209
Description
In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis.
Abbr
Leucine dehydrogenase (Bacillus sp.)
Source
Bacillus sp.
Applications
This enzyme is useful for enzyme determination of L-leucine and the activity of leucine amino-peptidase.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 1.4.1.9
Activity
GradeⅡ 20U/mg-solid or more (containing approx. 70% of stabilizers)
CAS No.
9082-71-7
Contaminants
Leucylpeptide decomposing enzymes (Leu-Val) < 1.0×10⁻²% (Leu-Gly-Gly) < 1.0×10⁻²% NADH oxidase < 1.0×10⁻²%
Molecular Weight
245 kDa
pH Stability
pH 5.5-10.5 (25°C, 20hr)
Michaelis Constant
1.0×10⁻³M (L-Leucine), 3.9×10⁻⁴M (NAD⁺), 3.5×10⁻⁵M (NADH), 3.1×10⁻⁴M [α-Ketoisocaproate (α-KIC)], 2.0×10⁻¹M (NH₃)
Structure
6 subunits per mol of enzyme
Optimum pH
10.5-10.8 (L-Leu→α-KIC), 9.4 (α-KIC→L-Leu)
Optimum temperature
above 70°C
Thermal stability
below 60°C (pH 6.9, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate
Inhibitors
Na₂S, Hg⁺⁺, Cu⁺⁺, Co⁺⁺, Mg⁺⁺, p-chloromercuribenzoate
Synonyms
EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH

"Leucine dehydrogenase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1715 Leucine dehydrogenase from Microorganism EC 1.4.1.9 9082-71-7 Microorganism Inquiry
NATE-1805 L-leucine Dehydrogenase (Crude Enzyme) EC 1.4.1.9 9082-71-7 E. coli Inquiry
NATE-1905 Native Bacillus stearothermophilus Leucine Dehydrogenase EC 1.4.1.9 9082-71-7 Bacillus stearothermophilus Inquiry
NATE-0391 Native Bacillus cereus L-Leucine Dehydrogenase EC 1.4.1.9 9082-71-7 Bacillus cereus Inquiry

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