Products

Online Inquiry

Name

Email *

Phone *

Company/Institution

Country or Region

Quantity

Services & Products Interested *

Project Description

Our Products Cannot Be Used As Medicines Directly For Personal Use.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Leucine dehydrogenase from Microorganism

inquiry

Cat No.

NATE-1715

Description

In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis.

Abbr

LEDH (Microorganism)

Source

Microorganism

Form

White powder, lyophilized

Enzyme Commission Number

EC 1.4.1.9

Activity

>500U/mg protein

CAS No.

9082-71-7

Molecular Weight

43 kDa (SDS-PAGE)

Isoelectric point

6.6

pH Stability

6.0~11.0 (25℃, 15hr)

Michaelis Constant

2.6×10^-4 M (NAD )
2.0×10^-3 M(L-Leucine)
6.8×10^-4 M(α-Ketoisocaproate)
4.2×10^-2 M (NH Cl)
2.3×10^-4 M (NADH)

Unit Definition

One unit will convert one micromole of L-Leucine to α-Ketoisocaproate per minute at pH 10.5 at 37℃.

Optimum pH

above11.0(L-Leu→α-K I C),
8.5(α-K I C→L-Leu)

Optimum temperature

55-60℃(L-Leu →α-K I C)
above 60℃(α-K I C→L-Leu)

Thermal stability

< 55℃(pH 7.0, 20min)

Storage

Store at -20°C.

Inhibitors

Hg2+

Synonyms

EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH

Notes

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

"Leucine dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1805	L-leucine Dehydrogenase (Crude Enzyme)	EC 1.4.1.9	9082-71-7	E. coli	Inquiry
NATE-1905	Native Bacillus stearothermophilus Leucine Dehydrogenase	EC 1.4.1.9	9082-71-7	Bacillus stearothermophilus	Inquiry
DIA-209	Native Bacillus sp. Leucine dehydrogenase	EC 1.4.1.9	9082-71-7	Bacillus sp.	Inquiry
NATE-0391	Native Bacillus cereus L-Leucine Dehydrogenase	EC 1.4.1.9	9082-71-7	Bacillus cereus	Inquiry

+ See More >>