Description
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3'-GMP and oligonucleotides with a 3'-GMP terminal group.
Abbr
RNase T1, Native (Aspergillus oryzae)
Source
Aspergillus oryzae
Applications
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies.
Form
ammonium sulfate suspension; Suspension in 2.8 M (NH4)2SO4 solution
Enzyme Commission Number
EC 3.1.27.3
Activity
300,000-600,000 units/mg protein
Unit Definition
One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.
Warnings
Protein determined by E1%/280
Synonyms
Ribonuclease T1; EC 3.1.27.3; guanyloribonuclease; Aspergillus oryzae ribonuclease; RNase N1; RNase N2; ribonuclease N3; ribonuclease U1; ribonuclease F1; ribonuclease Ch; ribonuclease PP1; ribonuclease SA; RNase F1; ribonuclease C2; binase; RNase Sa; guanyl-specific RNase; RNase G; RNase T1; ribonuclease guaninenucleotido-2'-transferase (cyclizing); ribonuclease N3; ribonuclease N1; 9026-12-4
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