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Ribonuclease T2 from Aspergillus oryzae, Recombinant

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Cat No.

NATE-1930

Description

Aspergillus oryzae Ribonuclease T2 is a member of the RNase T2 family of endonucleases that are present in a wide variety of microbial, plant and animal species. In contrast to Aspergillus oryzae Ribonuclease T1, which is an exclusively guanylic-acid specific endonuclease, all RNase T2-like enzymes are essentially base non-specific. However, RNase T2 endonucleases from different species can show slight base preferences. The fungal enzymes, including Aspargillus oryzae RNaseT2, show slight base preference in the following order: A>G>C, U. RNase T2 cleaves between the 3’-phosphate residue of one base and the 5’-OH residue of the adjacent nucleotide forming a 2’, 3’-cyclic phosphate intermediate followed by the generation of oligonucleotides with 3’-phosphate residues. This enzyme is also used as a non-mammalian source of RNase in various applications.

Source

Pichia pastoris

Species

Aspergillus oryzae

Form

Lyophilized powder

Enzyme Commission Number

EC 3.1.27.1

Activity

≥10,000 units per mg protein

Molecular Weight

36 kDa

Isoelectric point

Unit Definition

One unit will cause an increase in absorbance of 1.0 at 260 nm at 37°C, pH 4.5 in 15 minutes.

Optimum pH

4.5

Stability

Stable at 12-18 months at 2-8°C.

Storage

Store at 2-8°C.

Synonyms

Ribonuclease T2; RNase T2; Ribonuclease

"Rnase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1913	Ribonuclease from Rana Pipiens, Recombinant			E. coli	Inquiry
NATE-0655	Native Bovine Ribonuclease A	EC 3.1.27.5	9001-99-4	Bovine pancreas	Inquiry
NATE-0656	Native Bovine Ribonuclease B	EC 3.1.27.5	9001-99-4	Bovine pancreas	Inquiry
NATE-0657	Ribonuclease H from Escherichia coli, Recombinant	EC 3.1.4.34	9050-76-4	E. coli	Inquiry

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