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Ribonuclease T2 from Aspergillus oryzae, Recombinant
Cat No.
NATE-1930
Description
Aspergillus oryzae Ribonuclease T2 is a member of the RNase T2 family of endonucleases that are present in a wide variety of microbial, plant and animal species. In contrast to Aspergillus oryzae Ribonuclease T1, which is an exclusively guanylic-acid specific endonuclease, all RNase T2-like enzymes are essentially base non-specific. However, RNase T2 endonucleases from different species can show slight base preferences. The fungal enzymes, including Aspargillus oryzae RNaseT2, show slight base preference in the following order: A>G>C, U. RNase T2 cleaves between the 3’-phosphate residue of one base and the 5’-OH residue of the adjacent nucleotide forming a 2’, 3’-cyclic phosphate intermediate followed by the generation of oligonucleotides with 3’-phosphate residues. This enzyme is also used as a non-mammalian source of RNase in various applications.
Source
Pichia pastoris
Species
Aspergillus oryzae
Form
Lyophilized powder
Enzyme Commission Number
EC 3.1.27.1
Activity
≥10,000 units per mg protein
Molecular Weight
36 kDa
Isoelectric point
5
Unit Definition
One unit will cause an increase in absorbance of 1.0 at 260 nm at 37°C, pH 4.5 in 15 minutes.
Optimum pH
4.5
Stability
Stable at 12-18 months at 2-8°C.
Storage
Store at 2-8°C.
Synonyms
Ribonuclease T2; RNase T2; Ribonuclease
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1913 | Ribonuclease from Rana Pipiens, Recombinant | E. coli | Inquiry | ||
| NATE-0655 | Native Bovine Ribonuclease A | EC 3.1.27.5 | 9001-99-4 | Bovine pancreas | Inquiry |
| NATE-0656 | Native Bovine Ribonuclease B | EC 3.1.27.5 | 9001-99-4 | Bovine pancreas | Inquiry |
| NATE-0657 | Ribonuclease H from Escherichia coli, Recombinant | EC 3.1.4.34 | 9050-76-4 | E. coli | Inquiry |