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Hexosaminidase 3A from Thermotoga neapolitana, Recombinant

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Cat No.

NATE-1453

Description

Hexosaminidase, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

Abbr

Hexosaminidase 3A, Recombinant (Thermotoga neapolitana)

Source

E. coli

Species

Thermotoga neapolitana

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 3.2.1.52

CAS No.

9012-33-3

Molecular Weight

40.6 kDa

Purity

>90% by SDS-PAGE

Concentration

0.25 mg/mL

Specificity

Chitobiose and chitooligosaccharides

Optimum pH

7.0-8.0

Optimum temperature

65-75 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

EC 3.2.1.52; 9012-33-3; hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0779	Native Bovine β-N-Acetylglucosaminidase	EC 3.2.1.52	9012-33-3	Bovine kidney	Inquiry
NATE-0780	Native Canavalia ensiformis β-N-Acetylglucosaminidase	EC 3.2.1.52	9012-33-3	Canavalia ensiformis	Inquiry
NATE-0781	Native Human β-N-Acetylglucosaminidase	EC 3.2.1.52	9012-33-3	Human placenta	Inquiry
NATE-0782	β-N-Acetylglucosaminidase from Streptococcus pneumoniae, Recombinant	3.2.1.52	9012-33-3	E. coli	Inquiry

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