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Native Canavalia ensiformis β-N-Acetylglucosaminidase
Cat No.
NATE-0780
Description
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Abbr
β-N-Acetylglucosaminidase, Native (Canavalia ensiformis)
Source
Canavalia ensiformis
Applications
β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease 1. Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms.
Product Overview
At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.
Form
ammonium sulfate suspension. Suspension in 2.5 M (NH4)2SO4, pH 7.0
Enzyme Commission Number
EC 3.2.1.52
Activity
> 15 units/mg protein
CAS No.
9012-33-3
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25°C.
Storage
2-8°C
Synonyms
hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase; EC 3.2.1.52; 9012-33-3
Download
Download Datasheet"β-N-Acetylhexosaminidase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0779 | Native Bovine β-N-Acetylglucosaminidase | EC 3.2.1.52 | 9012-33-3 | Bovine kidney | Inquiry |
| NATE-0781 | Native Human β-N-Acetylglucosaminidase | EC 3.2.1.52 | 9012-33-3 | Human placenta | Inquiry |
| NATE-0782 | β-N-Acetylglucosaminidase from Streptococcus pneumoniae, Recombinant | 3.2.1.52 | 9012-33-3 | E. coli | Inquiry |
| NATE-0783 | Native Jack bean β-N-Acetylhexosaminidase | Jack bean | Inquiry |