Description
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.
Abbr
Heparinase III, Recombinant (Bacteroides eggerthii)
Species
Bacteroides eggerthii
Product Overview
Bacteroides Heparinase III cloned from Bacteroides eggerthii, also called Heparin Lyase III, is active on both heparin and heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. In stark contrast to the Flavobacterium heparinum Heparinase III that cleaves the glycosidic bond only between hexosamines and glucuronic acid residues, the Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. Flavobacterium heparinum Heparinase III is not active on an N-sulfoglucosamine with 6-sulfation, whereas the Bacteroides Heparinase III is active in the presence of 6-sulfation.
Form
100 mM NaCl, 20 mM Tris-HCl (pH 7.5 25°C), 1 mM Na2EDTA and 5 mM CaCl2.
Purity
> 95% determined by SDS-PAGE
Concentration
700 units/ml
Unit Definition
One unit is defined as the amount of enzyme that will liberate 1.0 μmol unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.0 in a total reaction volume of 100 μl.
Storage
at -80°C. Avoid repeated freeze/thaw cycles.
Synonyms
Heparinase; Heparin lyase; Heparin eliminase; Heparin-sulfate lyase; Heparin-sulfate eliminase; Heparitin-sulfate lyase; Heparinase I; Heparinase III; Heparin lyase II; Heparinase II
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