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Glucokinase from Human, recombinant

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Cat No.

NATE-1686

Description

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Abbr

GCK, Recombinant (Human)

Source

E. coli

Species

Human pancreatic

Form

Liquid

Enzyme Commission Number

EC 2.7.1.2

Activity

303 pmol/min/µg

CAS No.

9001-36-9

Molecular Weight

53.1 kDa

Purity

> 80% by SDS-PAGE

Concentration

1 mg/ml

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.

Storage

Store at -80°C. Avoid repeated freezing and thawing cycles.

Synonyms

EC 2.7.1.2; glucokinase; glucokinase (phosphorylating); 9001-36-9; GCK; FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; Hexokinase type IV; HK IV; Hexokinase-4; Hexokinase-D

Formulation

0.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0) with 50% glycerol.

"GCK" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1687	Glucokinase from Human, recombinant	EC 2.7.1.2	9001-36-9	E. coli	Inquiry
NATE-1696	Glucokinase from Human, recombinant	EC 2.7.1.2	9001-36-9	E. coli	Inquiry
NATE-1697	Glucokinase from Human, recombinant	EC 2.7.1.2	9001-36-9	E. coli	Inquiry
NATE-1903	Native Zymomonas mobilis Glucokinase	EC 2.7.1.2	9001-36-9	Zymomonas mobilis	Inquiry

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