Description
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Enzyme Commission Number
EC 2.7.1.2
Activity
>350 U/mg protein
Contaminants
(as GlcK2 activity = 100 %)
Glucose-6-phosphate dehydrogenase < 0.01 %
Phosphoglucomutase < 0.01 %
6-Phosphogluconate dehydrogenase < 0.01 %
Hexose-6-phosphate isomerase < 0.01 %
Glutathione reductase < 0.01 %
Molecular Weight
ca. 32,000
Michaelis Constant
(60mM Tris-HCI buffer, pH 8.5, at 30 °C)
Glucose 0.1 mM
ATP 0.05 mM
Unit Definition
One unit of activity is defined as the amount of GlcK2 that forms 1 μmol of glucose 6-phosphate per minute at 30 °C.
Optimum temperature
70 °C
Thermal stability
No detectable decrease in activity up to 60 °C.
Storage
Stable at -20°C for at least one year
Synonyms
EC 2.7.1.2; glucokinase; glucokinase (phosphorylating); 9001-36-9; GCK; FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; Human pancreatic glucokinase; pancreatic glucokinase
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