Description
The fructosyl peptide oxidase is an enzyme that catalyzes the reaction which produces a sugar osone (an α-keto aldehyde), a peptide, and hydrogen peroxide by oxidative cleaving, in the presence of oxygen molecules, the C--N bond in the ketose derivative produced by Amadori rearrangement of glucosylamine produced by the reaction between the hemiacetal of glucose and the N-terminal amino group of a peptide.
Abbr
Fructosyl-peptide Oxidase, Recombinant (E. coli)
Appearance
Yellow lyophilizate
Enzyme Commission Number
EC 1.5.3
Activity
> 4 U/mg lyophilizate
Molecular Weight
ca. 60 kDa
Michaelis Constant
3.4 x 10^-3 M (fructosyl-valyl-histidine) 4.4 x 10^-3 M (fructosyl-glycine) 8.9 x 10^-3 M (Nε-fructosyl-lysine)
Structure
monomer of 52 kDa (SDS-PAGE)
Specificity
fructosyl-valyl-histidine (100), fructosyl-glycine (53), Nε-fructosyl-lysine (84)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 8.0.
Optimum temperature
35–42°C
Thermal stability
below 45°C
Stability
Stable at 37°C for at least one month
Stabilizers
EDTA, glutamate
Synonyms
Fructosyl-peptide : oxygen oxidoreductase; EC 1.5.3; Fructosyl-peptide Oxidase
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