Description
In enzymology, a feruloyl esterase (EC 3.1.1.73) is an enzyme that catalyzes the chemical reaction: feruloyl-polysaccharide + H2O rightleftharpoons ferulate + polysaccharide. Thus, the two substrates of this enzyme are feruloyl-polysaccharide and H2O, whereas its two products are ferulate and polysaccharide. This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds.
Abbr
Feruloyl esterase, Recombinant (Acetivibrio cellulolyticus)
Source
Acetivibrio cellulolyticus
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.1.73
Molecular Weight
32631.3 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of ferulic acid per minute from 0.187 mM methyl ferulate in 50 mM sodium phosphate buffer, pH 6.5, at 37°C, and at 335 nm, and using a molar extinction coefficient of 14000 M-1cm-1.
Optimum temperature
> 37°C
Storage
Store at 4°C (shipped at room temperature)
Synonyms
feruloyl esterase; ferulic acid esterase (FAE); hydroxycinnamoyl esterase; hemicellulase accessory enzyme; cinnamoyl ester hydrolase (cinnAE); EC 3.1.1.73; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase