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D(-)-Luciferin

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Cat No.

CSUB-0354

Applications

Substrate for firefly luciferase with a Km of approx 2 μM.Used for the luminometric determination of Luc activity in cell extracts. Natural substrate of luciferase from firefly. Used together with firefly luciferase for the determination of ATP using bioluminescence.

CAS No.

2591-17-5

Molecular Weight

280.32

Storage

-20°C

Preparation Instructions

Working concentration: For the assay of medium concentrations of ATP (10-9 to 10-6 M in the assay cuvette), use 35 to 70 μM D(-)-Luciferin. For the assay of low concentrations of ATP (10-13 to 10-8 M in the assay cuvette), use 350 μM D(-)-Luciferin. For the assay of metabolites convertible to ATP or enzymes which produce ATP, the literature suggests concentrations of D(-)-Luciferin from 35-359 μM.Working solution: Preparation of D(-)-Luciferin solutionTo minimize handling of the unstable compound, prepare a D(-)-Luciferin solution at the approximate concentration desired, then adjust it to the exact concentration on the basis of absorbance at 327 nm. (The absorptivity of D(-)-Luciferin at 327 nm is 18.2 mmol-1 x l x cm-1).For instance, to prepare a 700 M solution of D()-Luciferin:• Add 1.5 mg of D(-)-Luciferin to 5 ml of 70 mM Tris-acetate, pH 7.75 [theoretical concentration = 1.07 mM]• Dilute a portion of that stock solution 20-fold with buffer.• Read the absorbance at 327 nm.• Add buffer to the stock so that a 20-fold dilution gives A327 of 0.637 (concentration of 20-fold dilution = 35 μM; concentration of stock = 700 μM)

Synonyms

(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid; 4, 5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid; D-Luciferin; Firefly Luciferin

Molecular Formula

C11H8N2O3S2

MDL Number

MFCD00042929

Shipping Conditions

dry ice

Beilstein Registry Number

30484

Substrates

Luciferase

"Luciferase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0422	Native Photinus pyralis (firefly) Luciferase	EC 1.13.12.7	9014-00-0	Photinus pyralis (firefly)	Inquiry
NATE-0423	Native Vibrio fischeri (Photobacterium f) Luciferase	EC 1.13.12.7	9014-00-0	Vibrio fischeri (Photobacterium f)	Inquiry
NATE-0424	Luciferase from Photinus pyralis (firefly), Recombinant	EC 1.13.12.7	61970-00-1	E. coli	Inquiry
NATE-1253	Luciferase from E. coli, Recombinant	EC 1.13.12.7		E. coli	Inquiry

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