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Luciferase from E. coli, Recombinant
Cat No.
NATE-1253
Description
Luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Abbr
Luciferase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
White lyophilizate
Enzyme Commission Number
EC 1.13.12.7
Activity
> 1.0 x 10^9 relative light units (RLU)/mg lyophilizate Specific activity: 1.4 x 10^11 RLU/mg purified protein
Molecular Weight
ca. 60 kDa
pH Stability
6.0–9.0
Michaelis Constant
1.9 x 10^-4 M (ATP) 1.5 x 10^-4 M (D-luciferin)
Structure
monomer of ca. 60 kDa (SDS-PAGE)
Unit Definition
One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8.
Optimum pH
7.0–8.5
Thermal stability
below ca. 40°C
Stability
stable at 25°C for at least 5 days (liquid form)
Storage
at -20°C
Synonyms
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1
Download
Download Datasheet"Luciferase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0422 | Native Photinus pyralis (firefly) Luciferase | EC 1.13.12.7 | 9014-00-0 | Photinus pyralis (firefly) | Inquiry |
| NATE-0423 | Native Vibrio fischeri (Photobacterium f) Luciferase | EC 1.13.12.7 | 9014-00-0 | Vibrio fischeri (Photobacterium f) | Inquiry |
| NATE-0424 | Luciferase from Photinus pyralis (firefly), Recombinant | EC 1.13.12.7 | 61970-00-1 | E. coli | Inquiry |
| NATE-1254 | Biotinylated Luciferase from E. coli, Recombinant | EC 1.13.12.7 | E. coli | Inquiry |