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Luciferase from E. coli, Recombinant

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Cat No.

NATE-1253

Description

Luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.

Abbr

Luciferase, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Appearance

White lyophilizate

Enzyme Commission Number

EC 1.13.12.7

Activity

> 1.0 x 10^9 relative light units (RLU)/mg lyophilizate Specific activity: 1.4 x 10^11 RLU/mg purified protein

Molecular Weight

ca. 60 kDa

pH Stability

6.0–9.0

Michaelis Constant

1.9 x 10^-4 M (ATP) 1.5 x 10^-4 M (D-luciferin)

Structure

monomer of ca. 60 kDa (SDS-PAGE)

Unit Definition

One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8.

Optimum pH

7.0–8.5

Thermal stability

below ca. 40°C

Stability

stable at 25°C for at least 5 days (liquid form)

Storage

at -20°C

Synonyms

Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1

"Luciferase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0422	Native Photinus pyralis (firefly) Luciferase	EC 1.13.12.7	9014-00-0	Photinus pyralis (firefly)	Inquiry
NATE-0423	Native Vibrio fischeri (Photobacterium f) Luciferase	EC 1.13.12.7	9014-00-0	Vibrio fischeri (Photobacterium f)	Inquiry
NATE-0424	Luciferase from Photinus pyralis (firefly), Recombinant	EC 1.13.12.7	61970-00-1	E. coli	Inquiry
NATE-1254	Biotinylated Luciferase from E. coli, Recombinant	EC 1.13.12.7		E. coli	Inquiry

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