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Carboxylesterase 1D from Mouse, Recombinant

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Cat No.

NATE-1633

Description

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Abbr

CES1D, Recombinant (Mouse)

Source

Insect cell (Baculovirus) and fused to His-tag at N-terminus

Species

Mouse

Form

Liquid

Activity

> 80,000 pmol/min/ug

Molecular Weight

60.9 kDa

Purity

> 90% by SDS-PAGE

Concentration

0.5 mg/ml

Unit Definition

Defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37°C.

Storage

Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.

Synonyms

Ces1d; Carboxylesterase 3; FAEE synthase; TGH; Ces3

Endotoxin Level

< 1 EU/μg

Formulation

Sterile Filtered lyophilized (freeze-dried) powder from 50 mM Tris, 100 mM NaCl, pH 8.0.

"Esterase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1916	Carboxylesterase 1 isoform c from Human, Recombinant	EC 3.1.1.1	9016-18-6	Baculovirus infected BTI insect cells	Inquiry
NATE-1915	Carboxylesterase 1 isoform b from Human, Recombinant	EC 3.1.1.1	9016-18-6	Baculovirus infected BTI insect cells	Inquiry
NATE-0229	Esterase Isoenzyme 1 from Porcine liver, Recombinant	EC 3.1.1.1	9016-18-6	E. coli	Inquiry
NATE-0230	Esterase Isoenzyme 2 from Porcine liver, Recombinant	EC 3.1.1.1	9016-18-6	E. coli	Inquiry

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