Description
Acetyl-CoA Carboxylase (ACC) regulates the metabolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation. The Acetyl-CoA Carboxylase enzymes are activated by Citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Acetyl-CoA Carboxylase 1 is essential for breast cancer and prostrate cancer cell survival. Because of thier roles in fatty acid metabolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and metabolic syndrome disorders.
Abbr
ACC1, Recombinant (Human)
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Form
Supplied as a solution in 50 mM Tris-HCl, pH 8.0, 275 mM NaCl, 10% glycerol, 1 mM EDTA and 2 mM DTT.
Enzyme Commission Number
EC 6.4.1.2
Activity
> 20 units/μg protein
Molecular Weight
292.5 kDa
Unit Definition
One unit will cause the carboxylation of 1.0 picomole of acetyl-CoA per minute at pH 7.5 at 30 °C.
Storage
Store at -70°C. Avoid multiple freeze-thaw cycles.
Synonyms
ACAC; ACACA; ACC1; ACC; ACCA; acetyl-CoA carboxylase alpha; acetyl coenzyme A carboxylase; acetyl-CoA carboxylase
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