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Native Microorganism Glycerol Kinase

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Activity

Size

Price

Qty

Activity30 U/mg

Size20 KU

Price$490

Qty

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Cat No.

DIA-149

Description

The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.

Abbr

GK (Microorganism)

Alias

Source

Microorganism

Applications

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.

Appearance

White amorphous powder, lyophilized

Form

Freeze dried powder

Enzyme Commission Number

EC 2.7.1.30

Activity

GradeⅢ 30 U/mg-solid or more

CAS No.

9030-66-4

Contaminants

Catalase < 1.0×10⁻¹% NADH oxidase < 1.0×10⁻³% Adenosine triphosphatase < 1.0×10⁻³%

Molecular Weight

approx. 220 kDa (by gel filtration)

Isoelectric point

4.3

pH Stability

pH 5.5-10.0 (25°C, 20hr)

Michaelis Constant

9.4×10⁻⁵M (Glycerol), 1.3×10⁻⁵M (ATP), 2.1×10⁻³M (Dihydroxyacetone)

Structure

Four subunits of approx. 58,000

Optimum pH

Optimum temperature

70°

Thermal stability

below 65°C (pH 7.5, 30min)

Stability

Stable at-20°C

Inhibitors

p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺

Synonyms

glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 2.7.1.30

"GK" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0286	Native Bacillus stearothermophilus Glycerokinase	EC 2.7.1.30	9030-66-4	Bacillus stearothermophilus	Inquiry
NATE-0287	Native Cellulomonas sp. Glycerokinase	EC 2.7.1.30	9030-66-4	Cellulomonas sp.	Inquiry
NATE-0288	Native Escherichia coli Glycerokinase	EC 2.7.1.30	9030-66-4	Escherichia coli	Inquiry
NATE-0289	Native Streptomyces canus Glycerokinase	EC 2.7.1.30	9030-66-4	Streptomyces canus	Inquiry

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