Description
Phospholipase A2 (PLA2) hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. It aggressively attacks phospholipids in membranes of intact cells. PLA2 specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids.
Abbr
PLA2, Native (Bovine)
Form
lyophilized powder; Contains Tris buffer salts.
Enzyme Commission Number
EC 3.1.1.4
Activity
> 20 units/mg protein
Unit Definition
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37°C.
Warnings
Protein determined by biuret.
Pathway
Acyl chain remodelling of PC, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem
Function
calcium ion binding; phospholipase A2 activity
Synonyms
Phospholipases A2; EC 3.1.1.4; 9001-84-7; lecithinase A; phosphatidase; phosphatidolipase; phospholipase A; PLA2; Phosphatidylcholine 2-acylhydrolase; PLA2s