Products

Enzymes for Research, Diagnostic and Industrial Use

Products
Online Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

24 hour
Promise

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant

Cat No.
NATE-1289
Description
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Abbr
β-acetylglucosaminidase 73A, Recombinant (Clostridium perfringens)
Source
E. coli
Species
Clostridium perfringens
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Molecular Weight
24.6 kDa
Purity
>90% by SDS-PAGE
Concentration
0.25 mg/mL
Specificity
Peptidoglycan
Optimum pH
8
Optimum temperature
37 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase

"β-N-Acetylhexosaminidase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-0779 Native Bovine β-N-Acetylglucosaminidase EC 3.2.1.52 9012-33-3 Bovine kidney Inquiry
NATE-0780 Native Canavalia ensiformis β-N-Acetylglucosaminidase EC 3.2.1.52 9012-33-3 Canavalia ensiformis Inquiry
NATE-0781 Native Human β-N-Acetylglucosaminidase EC 3.2.1.52 9012-33-3 Human placenta Inquiry
NATE-0782 β-N-Acetylglucosaminidase from Streptococcus pneumoniae, Recombinant 3.2.1.52 9012-33-3 E. coli Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

0
Click unfold / close
Inquiry Basket
Delete selected Quote Check Out
Decide to move out of the shopping cart?
Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product