Official Full Name
NADH Peroxidase
Background
The presumed function of NADH peroxidase is to inactivate H2O2 generated within the cell, for example by glycerol-3-phosphate oxidase during glycerol metabolism or dismutation of superoxide, before the H2O2 causes damage to essential cellular components.
Synonyms
NADH:hydrogen-peroxide oxidoreductase; DPNH peroxidase; NAD peroxidase; diphosphopyridine nucleotide peroxidase; NADH-peroxidase; nicotinamide adenine dinucleotide peroxidase; NADH2 peroxidase; EC 1.11.1.1
NADH peroxidase is an enzyme found in bacteria, fungi and plants that catalyzes the reduction of hydrogen peroxide (H2O2) using reduced nicotinamide adenine dinucleotide (NADH) as a source of electrons. This enzyme plays an important role in the antioxidant defense system of cells, protecting them from damage caused by oxidative stress and reactive oxygen species (ROS).
Structure
NADH peroxidase is a flavoprotein belonging to the family of peroxidases, enzymes that catalyze the reduction of H2O2 to water using a variety of electron donors. The enzyme consists of two identical subunits, each containing a FAD cofactor and a catalytic site composed of two histidine residues and a tyrosine residue. The mechanism of NADH peroxidase involves the transfer of electrons from NADH to the FAD cofactor, which is reduced to FADH2. FADH2 then transfers an electron to the H2O2 substrate, which is converted to water. The whole reaction can be expressed as:
H2O2 + 2 NADH + 2H+ → 2H2O + 2NAD+
Mechanism
NADH peroxidase is an important component of the cellular antioxidant defense system that protects cells from oxidative stress and damage caused by ROS, highly reactive molecules that can damage cellular components such as DNA, proteins and lipids. ROS are produced by normal cellular metabolism as well as by environmental stressors such as UV radiation, cigarette smoke and pollution. NADH peroxidase is involved in the reduction of H2O2, which is one of the major ROS produced in cells. If H2O2 is not effectively eliminated, it can react with iron and other transition metals to form highly reactive hydroxyl radicals, which can cause serious damage to cellular components.
Biological function
NADH eliminates potentially toxic hydrogen peroxide under aerobic growth conditions and represents an enzymatic defense against H2O2-mediated oxidative stress. Second, the enzyme provides an additional mechanism for regenerating NAD+ necessary for the strict fermentation metabolism of the organism. The enzyme may also prevent exogenous H2O2 and contribute to bacterial virulence.
NADH peroxidase and disease
Studies of NADH peroxidase have revealed its potential involvement in a variety of human diseases. For example, NADH peroxidase produced by Helicobacter pylori, a bacterium colonizing the human stomach, has been linked to the development of gastric cancer and chronic gastritis. NADH peroxidase is also thought to be associated with the development of fungal infections, particularly in immunocompromised patients. Certain fungal species, such as Candida albicans, secrete NADH peroxidase, which is involved in detoxifying ROS produced by the host immune response. inhibition of NADH peroxidase activity has been shown to increase the susceptibility of these fungi to oxidative stress, suggesting that it may be a potential target for antifungal therapy.
Conclusion
NADH peroxidase is an important enzyme that plays a key role in the cellular antioxidant defense system, protecting cells from oxidative stress and ROS damage. It has many potential applications in biotechnology and medicine, such as the removal of H2O2 from food and textile products, and the prevention and treatment of diseases related to oxidative stress. Studies on NADH peroxidase have also revealed its possible involvement in the development of various human diseases, such as bacterial and fungal infections. The development of specific NADH peroxidase inhibitors and activators may provide new therapeutic approaches to modulate the activity of this enzyme in a disease-specific manner and may lead to new insights into the regulation of cellular metabolism and signaling pathways.