Description
ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues. ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.
Abbr
ZAP70, Recombinant (Human)
Alias
ZAP70; SRK; TZK; ZAP-70; STD
Source
baculovirus infected insect cells (Histidine tagged)
Applications
Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.
Product Overview
> 90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, > 150 U/mg
Form
buffered aqueous glycerol solution
Molecular Weight
mol wt 70 kDa
Unit Definition
One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37°C at pH 7.0.
Buffer
Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.
Pathway
Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Generation of second messenger molecules, organism-specific biosystem; Immune System, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem; Natural killer cell mediated cytotoxicity, organism-specific biosystem; Natural killer cell mediated cytotoxicity, conserved biosystem
Function
ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity
Synonyms
ZAP-70 Tyrosine Kinase; Zeta-chain-associated protein kinase 70; ZAP-70; SRK; STCD; STD; TZK; ZAP70
Download Datasheet