Description
In enzymology, a xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1.151) is an enzyme that catalyzes the chemical reaction: xyloglucan + H2O rightleftharpoons xyloglucan oligosaccharides. Thus, the two substrates of this enzyme are xyloglucan and H2O, whereas its product is xyloglucan oligosaccharides. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds.
Abbr
XEG, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum F7/YS
Form
Supplied in a glycerol solution
Enzyme Commission Number
EC 3.2.1.151
Molecular Weight
82100 Da
Purity
>95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of glucose-reducing-sugar equivalents per minute from xyloglucan in 50 mM phosphate buffer, pH 6.5, at 60°C.
Optimum pH
7 (stable from 6 - 8)
Optimum temperature
60°C (stable up to 65°C)
Storage
Store at -20°C (shipped at ambient temperature)
Preparation Instructions
Centrifuge briefly if possible to remove glycerol solution from lid before use.
Synonyms
xyloglucan-specific endo-beta-1,4-glucanase; EC 3.2.1.151; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase; XEG; XH; xyloglucan endo-beta-1,4-glucanase; xyloglucanase; xyloglucanendohydrolase; 1,4-beta-D-glucan glucanohydrolase
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