Vaccinia virus capping enzyme is a heterodimer of D1 (844-aa) and D12 (287-aa) polypeptides that executes all three steps in m7GpppRNA synthesis. The D1 subunit comprises an N-terminal RNA triphosphatase (TPase)–guanylyltransferase (GTase) module and a C-terminal guanine-N7-methyltransferase (MTase) module.
Form
Colorless clear liquid
Activity
9-11U/ul
Contaminants
RNase-free.
DNase-free.
Host Cell DNA : <100pg/ul
Purity
>95% by SDS-PAGE
Storage
The product is stored at -20°C. Avoid repeated freeze-thaw cycles.
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