Description
UDG (Uracil-DNA Glycosylase) catalyses and hydrolyses uracil base and N- glycosidic bond of the sugar phosphate skeleton which belong to single or double-stranded DNA containing dU. Thus, free uracils are released and the base-free sites are dissociated easily by hydrolysis. Heat-labile UDG steming from psychrophilic marine bacterium, is sensitive to high temperature and therefore inactivates enzymes irreversibly when temperature over 50°C, which is suitable for PCR/QPCR, RT- PCR /RT-QPCR system.
Species
Psychrophilic marine bacterium
Unit Definition
One unit (U) is defined as the amount of enzyme that releases 1 nmol of uracils from the DNA strand (containing dU) within 1 hour at 37°C in the reaction system containing 70 mM of Tris - HCl, pH 7.5, 10 mM of NaCl, 1 mM of EDTA, 100 μg/ml of BSA reaction liquid.
Buffer
20 mM Tris-HCl, pH 8.0@ 25°C
0.1 mM EDTA
100 mM KCl
1 mM DTT
50% Glycerol (v/v)
0.5% NP-40 (v/v)
0.5% Tween-20 (v/v)
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