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Universal Protease Substrate

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Cat No.

CSUB-0520

Applications

PrincipleBy treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.

Form

lyophilized

Concentration

1 mg/mL

Storage

-20°C

Preparation Instructions

Working concentration: Approximately 1 mg/mlStorage conditions (working solution): -15 to -25 °CAn aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.

Synonyms

Substrate of protease; casein; resorufin-labeled; protease substrate, universal

Shipping Conditions

dry ice

Substrates

Protease

"Protease" Total Products Page

Enzymes

Catalog	Product Name	CAS No.	Source	Price
NATE-0631	Native Aspergillus oryzae Protease	9001-92-7	Aspergillus oryzae	Inquiry
NATE-0630	Protease S from Pyrococcus furiosus, Recombinant		Bacillus sp.	Inquiry
NATE-0629	Native Rhizopus sp. Protease	9001-92-7	Rhizopus sp.	Inquiry
NATE-0628	Native Bovine Protease	9001-92-7	bovine pancreas	Inquiry

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