Description
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-carbon bond, releasing phenol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme also slowly catalyses similar reactions with D-tyrosine, S-methyl-L-cysteine, L-cysteine, L-serine and D-serine.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
β-tyrosinase; L-tyrosine phenol-lyase (deaminating)
Reaction
L-tyrosine + H2O = phenol + pyruvate + NH3 (overall reaction); (1a) L-tyrosine = phenol + 2-aminoprop-2-enoate; (1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous); (1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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