Description
In enzymology, an alpha,alpha-phosphotrehalase (EC 3.2.1.93) is an enzyme that catalyzes the chemical reaction: alpha,alpha-trehalose 6-phosphate + H2O ↔ D-glucose + D-glucose 6-phosphate. Thus, the two substrates of this enzyme are alpha,alpha'-trehalose 6-phosphate and H2O, whereas its two products are D-glucose and D-glucose 6-phosphate. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. This enzyme participates in starch and sucrose metabolism.
Abbr
α,α-phosphotrehalase, Recombinant (E. coli)
Source
Escherichia coli str. K-12 substr. W3110
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.93
Molecular Weight
67657.8 Da
Purity
>95 % as judged by SDS-PAGE
Concentration
3472.9 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of pNP per minute from pNP-α-D-glucopyranoside (5 mM) in 50 mM sodium acetate buffer, pH 6.0, containing 1 mg/mL BSA and 1 M sodium chloride, at 37 °C, and using an extinction coefficient of 18000 M-1cm-1. The enzyme should be diluted in 1 mg/mL BSA.
Optimum temperature
> 37°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
α,α-Trehalose-6-phosphate phosphoglucohydrolase; α,α-phosphotrehalase; phosphotrehalase; alpha,alpha-trehalose-6-phosphate phosphoglucohydrolase; alpha,alpha-phosphotrehalase
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