Description
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
Abbr
TG, Recombinant (Cynomolgus)
Applications
Labeling, immobilisation, conjugation and modification of proteins.
Appearance
White lyophilized solid.
Form
The purified transglutaminase is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, contains maltodextrin.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 2000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
Molecular Weight
84 kDa (monomer), 168 kDa (homodimer)
Purity
> 95 % by SDS-PAGE under reducing conditions
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Storage
Store at -20°C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
Reconstitution
Add at least the volume of H2O the protein is lyophilized from to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
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