Description
Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called "microsomal antigen" has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.
Abbr
TPO, Recombinant (Human)
Applications
Western-Blot
Product Overview
Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass. The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Molecular Weight
92,872 Da
Purity
Greater than 95% as determined by SDS-PAGE.
Concentration
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Buffer
TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
Synonyms
Thyroid peroxidase; EC 1.11.1.8; TPO; MSA; TPX; iodotyrosine deiodase; iodinase; iodoperoxidase (heme type); iodide peroxidase-tyrosine iodinase; iodotyrosine deiodinase; monoiodotyrosine deiodinase; thyroperoxidase; tyrosine iodinase; iodide peroxidase
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