Description
Thrombin is a serine protease that in humans is encoded by the F2 gene. Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade, which ultimately results in the reduction of blood loss. Thrombin in turn acts as a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions.
Abbr
Thrombin, Recombinant (Human)
Alias
Thrombin; factor IIa
Applications
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.
Product Overview
Thrombin is the final coagulation protease in regard to hemostasis, promoting both pr ocoagulant and anticoagulant effects.
Form
aqueous solution; supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride
Enzyme Commission Number
EC 3.4.21.5
Pathway
Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Blood Clotting Cascade, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem
Function
calcium ion binding; growth factor activity; peptidase activity; protein binding; receptor binding; contributes_to receptor binding; serine-type endopeptidase activity; thrombospondin receptor activity
Synonyms
thrombin; EC 3.4.21.5; fibrinogenase; thrombase; thrombofort; topical; thrombin-C; tropostasin; activated blood-coagulation factor II; blood-coagulation factor IIa; factor IIa; E thrombin; β-thrombin; γ-thrombin
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