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Terminal Transferase from Calf, Recombinant
Cat No.
NATE-1926
Description
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
Source
E. coli
Species
Calf thymus
Enzyme Commission Number
EC 2.7.7.31
Activity
42,000 units/mg
CAS No.
9027-67-2
Molecular Weight
58000 daltons
Concentration
20,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol dTTP into acid-insoluble material in a total reaction volume of 50ul in 1 hour at 37°C using d(A)18 as primer.
Storage
at -20°C
Synonyms
DNA nucleotidylexotransferase; terminal deoxyribonucleotidyltransferase; terminal addition enzyme; addase; deoxynucleotidyl terminal transferase; deoxyribonucleic acid nucleotidyltransferase; deoxyribonucleic nucleotidyltransferase; terminal deoxynucleotide transferase; TdT; EC 2.7.7.31; 9027-67-2
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0692 | Native Calf Terminal Transferase | EC 2.7.7.31 | 9027-67-2 | Calf thymus | Inquiry |
| EXWM-3242 | DNA nucleotidylexotransferase | EC 2.7.7.31 | 9027-67-2 | Inquiry |