Description
Taurine dioxygenase (TauD) is a Fe(II) and α-ketoglutaric aciddependent dioxygenase, which enables E.coli to use taurine as a sulfur source. The oligomeric state of the enzyme from E.coli is reported both as a dimer and tetramer. It contains a 2-His, 1- carboxylate facial triad, which is present in most members of the family of Fe(II)⁄ α-KG-dependent enzymes. TauD catalyzes the conversion of the amino acid taurine (2-aminoethane-1-sulfonic acid) to sulfite and aminoacetaldehyde.
Abbr
TauD, Recombinant (E. coli)
Enzyme Commission Number
EC 1.14.11.17
Molecular Weight
34.3 kDa, His tagged
Unit Definition
One unit is the amount of enzyme that converts 1 µmole of taurine to sulfite per minute at pH 6.9 at 30 °C.
Storage
Aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
Synonyms
TauD; 2-aminoethanesulfonate dioxygenase; Sulfate starvation-induced protein 3; SSI3; alpha-ketoglutarate-dependent taurine dioxygenase
Formulation
0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.
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