Description
The enzyme is involved in spheroidenone biosynthesis and in 2,2'-dioxospirilloxanthin biosynthesis. The enzyme from Rhodobacter sphaeroides contains heme at its active site.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
CrtA; acyclic carotenoid 2-ketolase; spirilloxanthin monooxygenase; 2-oxo-spirilloxanthin monooxygenase
Reaction
(1) spheroidene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = spheroiden-2-one + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction); (1a) spheroidene + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2-hydroxyspheroidene + oxidized ferredoxin [iron-sulfur] cluster + H2O; (1b) 2-hydroxyspheroidene + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2,2-dihydroxyspheroidene + oxidized ferredoxin [iron-sulfur] cluster + H2O; (1c) 2,2-dihydroxyspheroidene = spheroiden-2-one + H2O (spontaneous); (2) spirilloxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = 2-oxospirilloxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction); (2a) spirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2-hydroxyspirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O; (2b) 2-hydroxyspirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2,2-dihydroxyspirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O; (2c) 2,2-dihydroxyspirilloxanthin = 2-oxospirilloxanthin + H2O (spontaneous); (3) 2-oxospirilloxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = 2,2'-dioxospirilloxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction); (3a) 2-oxospirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2'-hydroxy-2-oxospirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O; (3b) 2'-hydroxy-2-oxospirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2H+ = 2',2'-dihydroxy-2-oxospirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O; (3c) 2',2'-dihydroxy-2-oxospirilloxanthin = 2,2'-dioxospirilloxanthin + H2O (spontaneous);
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Download Datasheet