Description
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Applications
1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
Enzyme Commission Number
EC 3.4.21.1
Activity
>1500 unit/mg protein
Molecular Weight
26,950 Da
Storage
Recombinant Chymotrypsin lyophilized should be stored under 2°C-8°C in sealed container. It is stable within 24 months. After dissolved, it should be stored under -20°C. It is stable within 24 months and above 90% activity remained after 3 times repeated freezing and thawing.
Preparation Instructions
For disolving buffer, it is 50mM HAC,to get the terminal concentration of 0.5μg/μl, used once time or stored below -20°C. For the digestion buffer, in general, it is 100mM NH4HCO3 containing 5mM CaCl2, at 30°C, to dissolve the aim protein and then added chymotrypsin (in general, W:W=1:20-1:100). Self digestion may occur if temperatures above 37°C.
Synonyms
EC 3.4.21.1; chymotrypsins A and B; alpha-chymar ophth; avazyme; chymar; chymotest; enzeon; quimar; quimotrase; alpha-chymar; alpha-chymotrypsin A; alpha-chymotrypsin; Chymotrypsin; α-Chymotrypsin; Sequencing Grade Recombinant Chymotrypsin; Sequencing Grade Chymotrypsin
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