Description
Rhamnogalacturonan Lyase is an endo-1,4-α-rhamnogalacturonan lyase. This enzyme catalyses the following chemical reaction: Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Abbr
Rhamnogalacturonan Lyase, Recombinant (Cellvibrio japonicus)
Species
Cellvibrio japonicus
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.-
Molecular Weight
79.6 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Rhamnogalacturonan
Optimum temperature
45 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
endo-1,4-α-rhamnogalacturonan lyase; EC 4.2.2.-
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