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Reverse Transcriptase from Moloney Murine Leukemia Virus, Recombinant
Cat No.
NATE-0660
Description
This Reverse Transcriptase has exceptionally strong strand displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile and well-suited for applications requiring full-length cDNA such as preparation of cDNA libraries and other techniques involving first strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR). It can be used for performing a reverse transcription reaction with any RNA template including GC-rich templates and RNAs with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H Minus. Because of the excellent extension capability of PrimeScript Reverse Transcriptase, preparation of cDNA can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
Abbr
RT, Recombinant (Moloney Murine Leukemia Virus)
Alias
RT
Source
E. coli
Species
Moloney Murine Leukemia Virus
Applications
RT-PCR; First strand cDNA synthesis; cDNA probe preparation; Synthesis of cDNA libraries with a high proportion of full-length cDNAs.
Product Overview
1. Strong strand displacement and extension capability: Capable of synthesizing long and full-length cDNA molecules (up to 12 kb); 2. Preparation of cDNA at 42°C results in lower background and higher cDNA yields because the lower temperature does not promote RNA degradation; 3. Outstanding accuracy: Lowest error rate among five commercially available reverse transcriptases tested; 4. Highly specific: Low rates of non-specific annealing, even on incompletely denatured RNA; 5. Works on challenging templates: Excellent results even with GC-rich templates and templates with high levels of secondary structure 6. Highly sensitive: use less of your precious RNA samples; 7. Demonstrated success with reverse transcription reaction times of 30 min. (60 min. for longer transcripts).
Unit Definition
One unit is the amount of the enzyme that incorporates 1 nmol of [3H]dTTP in 10 minutes at 37°C, with poly (rA), oligo (dT) 12-18 as the primer-template.
Storage
Storage at-20°C
Buffer
5X Buffer (for cDNA synthesis) 250 mM Tris-HCl, pH 8.3 375 mM KCl 15 mM MgCl2 Reaction mixture for unit definition: 50 mM: Tris-HCl, pH 8.3 75 mM: KCl 8mM: MgCl2 10 mM: DTT 20 ug/mL: (ra)n (dT)12-18 0.5 mM: [3H]dTTP 0.1%: NP-40 Storage Buffer Composition 200 mM Tris-HCl, pH 7.8 100 mM NaCl 1 mM EDTA 1 mM DTT 50% Glycerol (v/v)
Synonyms
Reverse transcriptase; RT
Download
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