Description
The enzyme catalyses an essential step in fatty acid biosynthesis, the reduction of the 2,3-double bond in enoyl-acyl-[acyl-carrier-protein] derivatives of the elongating fatty acid moiety. The enzyme from the bacterium Escherichia coli accepts substrates with carbon chain length from 4 to 18. The enzyme from the bacterium Mycobacterium tuberculosis prefers substrates with carbon chain length from 12 to 24 carbons.
Species
Mycobacterium tuberculosis
Form
Liquid or Lyophilized powder
Enzyme Commission Number
EC 1.3.1.9
Molecular Weight
32.6 kDa
Purity
Greater than 90% as determined by SDS-PAGE.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Buffer
Tris-based buffer, 50% glycerol
Reaction
an acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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