Description
The enzyme is an anomer-inverting glucosidase that catalyses the hydrolysis of the α-glucosidic O-linkage of α,α-trehalose, releasing initially equimolar amounts of α- and β-D-glucose. It is widely distributed in microorganisms, plants, invertebrates and vertebrates.
Enzyme Commission Number
EC 3.2.1.28
Purity
Greater than 85% as determined by SDS-PAGE.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Buffer
Tris/PBS-based buffer, 6% Trehalose, pH 8.0