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Recombinant Clostridium Histolyticum Collagenase I
Cat No.
DIGS-254
Description
Collagenase I from Clostridium histolyticum is prepared by recombinant expression in Escherichia coli and has a molecular weight of approximately 126 kDa. It includes a histidine tag.
Collagen is not easily degraded by common proteases and only undergoes hydrolysis under high-temperature or extreme acidic/alkaline conditions. However, collagenase can specifically hydrolyze the triple helical structure of native collagen under physiological pH and temperature conditions.
Collagen is not easily degraded by common proteases and only undergoes hydrolysis under high-temperature or extreme acidic/alkaline conditions. However, collagenase can specifically hydrolyze the triple helical structure of native collagen under physiological pH and temperature conditions.
Source
E. coli
Species
Clostridium histolyticum
Applications
For hydrolysis of collagen.
Dissolution Buffer: Dissolve in 0.15 mol/L sodium chloride and 0.02 mol/L phosphate buffer, pH 7.0–7.4. After dissolution, aliquot and store at below -15°C.
Enzyme Digestion Buffer: 0.15 mol/L sodium chloride, 0.02 mol/L phosphate buffer (pH 7.2), and 0.1 mM calcium chloride, pH 7.0–7.4.
Dissolution Buffer: Dissolve in 0.15 mol/L sodium chloride and 0.02 mol/L phosphate buffer, pH 7.0–7.4. After dissolution, aliquot and store at below -15°C.
Enzyme Digestion Buffer: 0.15 mol/L sodium chloride, 0.02 mol/L phosphate buffer (pH 7.2), and 0.1 mM calcium chloride, pH 7.0–7.4.
Form
0.15 mol/L sodium chloride, 0.02mol/L phosphate buffer, pH 7.0~7.4; Dissolve and pack, store below -15°C.
Activity
≥0.1USP/mg
CAS_No
9001-12-1
Molecular Weight
126Da±10kDa
Purity
≥80%
Storage
The freeze-dried powder is stored below -20°C for 36 months. 0.15 mol/L sodium chloride, 0.02mol/L phosphate buffer, pH 7.0~7.4.
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