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Pyranose Oxidase from E. coli, Recombinant
Cat No.
NATE-1252
Description
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. P2O is a homotetrameric protein that contains covalently bound flavin adenine dinucleotide (FAD). The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide.
Abbr
P2O, Recombinant (E. coli)
Alias
P2O
Source
E. coli
Species
E. coli
Appearance
Yellow lyophilizate
Enzyme Commission Number
EC 1.1.3.10
Activity
> 3 U/mg lyophilizate
CAS No.
37250-80-9
Molecular Weight
ca. 290 kDa
pH Stability
3.5–11.0
Michaelis Constant
7.4 x 10^-4 M (D-glucose) 1.5 x 10^-2 M (1,5-anhydroglucitol)
Structure
4 subunits of 64 kDa (SDS-PAGE)
Specificity
D-glucose (100), 1,5-anhydroglucitol (22)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.0.
Optimum pH
6.5
Optimum temperature
55°C
Thermal stability
below 55°C
Storage
at -20°C
Stabilizers
Glutamate
Synonyms
pyranose oxidase; EC 1.1.3.10; glucose 2-oxidase; pyranose-2-oxidase; 37250-80-9; P2O
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1718 | Pyranose Oxidase from Microorganism | EC 1.1.3.10 | 37250-80-9 | Microorganism | Inquiry |
| NATE-0500 | Pyranose Oxidase from Coriolus sp., Recombinant | EC 1.1.3.10 | 37250-80-9 | E. coli | Inquiry |
| EXWM-0392 | pyranose oxidase | EC 1.1.3.10 | 37250-80-9 | Inquiry |