Description
Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys157 and Ile158. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.
Abbr
Pro-Urokinase, Recombinant (Human)
Molecular Weight
49.3 kDa
Unit Definition
1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.
Storage
Stable at –80°C for at least 1 year as supplied. Store reconstituted aliquots at –80°C. Avoid repeated freeze and thaw cycles.
Synonyms
Single chain Urokinase-type plasminogen activator; scuPA; Urokinase-type Plasminogen Activator uPA; PLAU
Reconstitution
Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at –80°C.
Formulation
0.5 mg/ml solution of protein in 25 mM sodium acetate, 50 mM NaCl, pH 5.0 containing 50% glycerol.
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