Description
In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction:3,4-dihydroxybenzoate + O2↔ 3-carboxy-cis,cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.
Abbr
PCD, Recombinant (Bacteria)
Applications
Useful for removal of protocatechuate derived from choline esterase determination.
Enzyme Commission Number
EC 1.13.11.3
Contaminants
NADPH oxidase < 0.01 % Alkaline phosphatase < 0.002 %
Molecular Weight
28 kD α subuit, 24 kD β subunit（SDS-PAGE）
Storage
1 - 10°C (do not freeze)
Synonyms
protocatechuate 3,4-dioxygenase; protocatechuate oxygenase; protocatechuic acid oxidase; protocatechuic 3,4-dioxygenase; protocatechuic 3,4-oxygenase; 9029-47-4; EC 1.13.11.3; PCD
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