Description
Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.
Abbr
PKG1B, Recombinant (Human)
Alias
PRKG1B; PRKGR1B; PKG1B
Source
baculovirus infected insect cells
Applications
Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.
Product Overview
> 95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution
Form
buffered aqueous glycerol solution
Activity
> 1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
Molecular Weight
mol wt 76 kDa (monomer)
Unit Definition
One unit will phosphorylate 1 micromole of VASPtide (RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.
Buffer
Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.
Pathway
Adaptive Immune System, organism-specific biosystem; Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Hemostasis, organism-specific biosystem; Immune System, organism-specific biosystem; Long-term depression, organism-specific biosystem; Long-term depression, conserved biosystem
Function
ATP binding; cGMP binding; cGMP-dependent protein kinase activity; calcium channel regulator activity; nucleotide binding; protein binding; protein serine/threonine kinase activity
Synonyms
Protein Kinase G Iβ; PRKG1B; PRKGR1B; PKG1B; cGMP-dependent protein kinase 1; cGKI-BETA
Download Datasheet