Description
In enzymology, a galacturan 1,4-alpha-galacturonidase (EC 3.2.1.67) is an enzyme that catalyzes the chemical reaction: (1,4-alpha-D-galacturonide)n + H2O → (1,4-alpha-D-galacturonide)n-1 + D-galacturonate. Thus, the two substrates of this enzyme are (1,4-alpha-D-galacturonide)n and H2O, whereas its two products are (1,4-alpha-D-galacturonide)n-1 and D-galacturonate. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds.
Abbr
Polygalacturonase 4A, Recombinant (Bacillus subtilis)
Species
Bacillus subtilis
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.67
Molecular Weight
51.5 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Polygalacturonans and pNPαGalUA
Optimum temperature
36.5 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
poly(1,4-alpha-D-galacturonide) galacturonohydrolase; exopolygalacturonase; poly(galacturonate) hydrolase; exo-D-galacturonase; exo-D-galacturonanase; exopoly-D-galacturonase; galacturan 1,4-alpha-galacturonidase; EC 3.2.1.67; Polygalacturonase
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