Description
In enzymology, a poly(alpha-L-guluronate) lyase (EC 4.2.2.11) is an enzyme that catalyzes the chemical reaction: Eliminative cleavage of polysaccharides containing a terminal alpha-L-guluronate group, to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
Poly α-guluronate lyase, Recombinant (Zobellia galactanivorans)
Species
Zobellia galactanivorans
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.11
Molecular Weight
29.1 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Sodium alginate
Optimum temperature
30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
poly(alpha-L-1,4-guluronide) exo-lyase; alginase II; guluronate lyase; L-guluronan lyase; L-guluronate lyase; poly-alpha-L-guluronate lyase; polyguluronate-specific alginate lyase; poly(alpha-L-guluronate) lyase; EC 4.2.2.11
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